Live Animal Use

K-12 Education
Post-Secondary Education

K-12 Education

In K-12 education, animals are first normally introduced as classroom pets, which can present issues with the care and welfare of different species. Schools also use chicks, frogs, fish, rats, mice, and insects in invasive genetics, physiology, psychology, and science fair projects, which raise even greater humane concerns. Sadly, animals used in live experiments at the pre-college level are not afforded legal protections under the Animal Welfare Act (AWA). However, organizations such as the National Science Teachers Association (NSTA) and National Association of Biology Teachers (NABT) have created specific recommendations for the use and care of animals in K-12 education to address this lack of regulation. The American Veterinary Medical Association (AVMA) encourages the use of alternatives to live animals for pre-college classroom instruction; it suggests that the same instructional goals can be accomplished through other activities such as web-based tutorials, mock physiology datasets, and observational field studies or trips. 

Animals in the Classroom/Classroom Pets  

Making the decision to introduce an animal into a classroom should never be taken lightly. Serious issues can arise, including improper animal care for specific species, mishandling by students, and neglect over weekends and holidays. Animals in classrooms can cause health concerns for students and financial burdens on teachers. The National Science Teaching Association offers extensive guidelines for classroom pets to help ensure that animals are properly cared for and treated humanely, responsibly, and ethically.     

One independent study found that some teachers believe classroom pets have educational, leadership, and character-building value. However, the same study identified the many challenges mentioned above. (American Humane Association, July 2015) Exposing children to animals can certainly provide enrichment to their lives, but there are many ways that teachers can introduce animals to their students without bringing them into the classroom. For example, teachers can take students outside to observe animals in their natural habitats, which also builds observational and data collection skills. Teachers can plan trips to animal sanctuaries or local wildlife refuges. They can also use webcams and schedule online virtual tours so animals can be seen enjoying natural behaviors in a habitat instead of being confined in a small cage or aquarium.  

Chick Hatching  

Some elementary schools hatch chicks as an annual science project. Sadly, chick hatching often results in the neglect, suffering, and death of these baby birds. Chick hatching can also be harmful to the health of young children participating in these projects. In fact, the Center for Disease Control recommends that children less than 5 years old do not have any contact with baby chicks, and that older individuals pay close attention to hygiene in order to prevent transmission of Salmonella. ​(Salmonella Outbreaks Linked to Backyard Poultry, 2022)​ 

Although the project is meant to teach students about embryonic growth, it is not necessary to use live birds to accomplish this. The lack of proper care and attention to eggs during the incubation period can result in chicks suffering deformities and/or death. Chicks are often not provided with veterinary care after they hatch, and once the project is completed, teachers then must find homes for the animals or have them euthanized. Animal shelters are overwhelmed with unwanted cats and dogs and are usually unable to accept chicks, and the birds are often not allowed in suburban neighborhoods. Farms rarely take the chicks because of the danger of infecting their flocks with disease, making it likely that the chicks will be killed if no home is available for them. A wonderful humane alternative that can be used in place of traditional chick hatching projects is the Hatching Kindness Project - a curriculum unit developed by Kansas-based farmed animal sanctuary Shy 38 Inc. and Animalearn. 

Check out our Chick Hatching Fact Sheet too!

Genetics Experiments  

Fruit flies are often used in high school biological research and genetics classes because they are small insects with a short life cycle, are cheap to purchase, and easy to keep in large numbers. In some biology classes, they are used to examine how organisms arise from a  fertilized egg. Following the experiment, the fruit flies are commonly killed using an alcohol solution. However, there have been instances where fruit flies were released outside of the science classroom environment and caused health risks in schools. Research shows that fruit flies can transfer germs from a dirty surface onto a clean one. Some of the bacteria they may carry include salmonella, E. coli, and listeria, which are all linked to food poisoning. ​(Black, Hinrichs, Barcay, & Gardner, 2018)​ Instead of using fruit flies for genetics, educators could instead use plants, like corn, to conduct such experiments. Many students ethically object to the unnecessary killing of any living being to study science, so they do not want to participate in these types of experiments.  

Science Fairs  

Science fairs are terrific ways for pre-college students to display their knowledge of science, but they do not need to include live animals. When selecting a topic to study for science fairs, students may be encouraged by teachers to use animals in experiments that involve physical pain or stress, psychological stress, surgical procedures, force-feeding, drug addiction, and/or radiation. Not only do these experiments often cause great harm to animals, but they also waste resources and animal life and produce little or no new information because they duplicate past studies. Since these students usually have no training in the humane care, use, and handling of animals, these projects can be especially harmful for the animals involved. Some science fairs, such as the prestigious International Science and Engineering Fair, have issued strict guidelines in regard to student use of animals, but other science fairs still encourage animal use.

Postsecondary Education

Most animals used at college, veterinary, and medical school levels are often in situations where more sophisticated, non-animal alternatives could be used instead. Many humane alternatives can replace live animals for those pursuing an advanced degree (i.e., physiology, psychology), as well as veterinary and medical training skills. In life sciences, such alternatives include ethically sourced animal cadavers, prosected human cadavers, computer software, virtual reality technology, and incredibly realistic mannequins and surgical simulators that “breathe” and “bleed.” Veterinary schools are replacing the harmful use of animals, such as terminal labs, with beneficial and therapeutic programs such as having students perform low cost spay/neuter services and are obtaining animal cadavers ethically from client donations and Educational Memorial Programs (EMPs).  

Physiology Training (Frog Pithing) 

“It was the most unrespectful [sic], tormenting experience of my life. I spent almost half an hour in the bathroom crying.”
— University student’s response after a frog pithing exercise

Live frogs are often used in undergraduate physiology courses (and sometimes in high school classrooms) to demonstrate certain physiological reactions and processes. (Medler, 2019) These animals (usually leopard frogs) are rendered brain-dead by inserting a blade into and across the mouth of a live and fully conscious frog and slicing the top of the head off. Sometimes the animals can be “double-pithed” by destroying the spinal cord as well with a thrust of the probe through the vertebrae. After students pith the frog, the gastrocnemius muscle (frog’s rear leg muscle) is dissected out of the body and hooked up to an electrical recording device so that students can observe and analyze how muscles react to electrical stimuli. (Balcombe, 2000)

When live animals are used in invasive teaching labs, some students have reported being disturbed and traumatized by these experiences, including those who have reported seeing frogs trying to escape from these procedures. Many students are opposed to pithing because they feel it is unethical to cause pain and death to live animals.  

An online program called Virtual Physiology is a viable alternative to frog pithing.

Psychology Training

In some postsecondary (and some high school) psychology classes, live rats are used to demonstrate classical and operant conditioning techniques and similar theories of learning. These animals are kept in a container or cage where they are trained to press a bar to obtain food, and similar activities. Many of the rats used in psychology classes die from neglect or are killed when the class is over. Some are given or sold to pet stores where they are typically used as food for snakes or other animals. Many students do not want to experiment on rats because they object to using them as disposable tools.  A more humane alternative to using rats and mice in psychology experiments is Sniffy the Virtual Rat program.  

Veterinary and Medical Education Training

Veterinary Education

Individuals who want to pursue a veterinary or medical degree without harming animals can do so. However, harmful animal use continues to be a supported practice in both the veterinary and medical education fields.

For those interested in veterinary careers, the American Association of Veterinary Medical Colleges (AAVMC) offers strict guidance for animal use as shown in its  Guidelines for the Use of Animals in Veterinary Education from October 2022. It notes, “Animal use should be guided by 4 Rs (replacement, reduction, refinement, and respect) and must be compliant with applicable laws and regulations.” Fortunately, several of the 33 U.S. veterinary schools no longer require terminal surgeries (labs that require the killing of healthy animals) in core courses, and many no longer require them in elective courses. To learn more about advances in veterinary education, you can read the AV Magazine 2021 Issue 1, which focuses on Veterinarians, especially “Veterinary Education Advances on pages 4-6.  

Medical Education

Today students who care about animals and are looking to pursue a medica degree are able to do so without compromising their ethical beliefs.  In 2016, it was reported that the University of Tennessee was the last remaining medical school in the United States and Canada vowing to end the use of live animal labs in their medical courses, moving to the utilization of models and simulations. (Wanshel, 2016) While this is significant progress for students looking to obtain a humane medical degree, it should be noted that animals continue to be used in more advanced training, including surgical and emergency medicine residencies. The 2002 American College of Surgeon's (ACS) Statement on the Use of Animals in Research, Education, and Teaching states, “The ACS believes that now and in the foreseeable future it is not possible to completely replace the use of animal and that the study of whole living organisms, tissues, and cells is an indispensable element of biomedical research, education, and teaching.”

A 2025 opinion piece featured on the ACS website counters the need for live tissue training (LTT) for medical students today. (Gonzalez-Fisher, 2025) This article advocates alternatives that respect animal welfare while advancing medical education.

To learn more about animals used in higher education and the alternatives, read “Dying To Learn – Exposing the supply and use of dogs and cats in higher education”.

REFERENCES

  1. Balcombe, J. (2000). The Use of Animals in Higher Education: Problems, Alternatives, & Recommendations. Humane Society Press, 53-54.

  2. Black, E., Hinrichs, G., Barcay, S., & Gardner, D. (2018, March 1). Fruit Flies as Potential Vectors of Foodborne Illness. Journal of Food Protection, 81(3), 509-514. Retrieved July 17, 2023, from https://doi.org/10.4315/0362-028X.JFP-17-255

  3. Gonzalez-Fisher, R. F. (2025, June 10). Jerry’s Ethical Framework Analyzes Nonhuman Animal Use in Surgical Skill Development. ACS Bulletin, 110(6). Retrieved 2025, June 16 from American College of Surgeons: https://www.facs.org/for-medical-professionals/news-publications/news-and-articles/bulletin/2025/june-2025-volume-110-issue-6/jerry-s-ethical-framework-analyzes-nonhuman-animal-use-in-surgical-skill-development/

  4. Meder, Scott (2019, January 29). Anesthetic MS-222 eliminates nerve and muscle activity in frogs used for physiology teaching laboratories. Advances in Physiology Education, 43(1). doi: https://doi.org/10.1152/advan.00114.2018

  5. Salmonella Outbreaks Linked to Backyard Poultry. (2022, November 10). Retrieved July 17, 2023, from Centers for Disease Control and Prevention: https://www.cdc.gov/salmonella/backyardpoultry-06-22/index.html

  6. Wanshel, E. (2016, July 6). Last US Medical School That Used Live Animals in Teaching Surgery Ends Practice. Retrieved 2025, June 16 from Huffington Post: https://www.huffpost.com/entry/live-animals-pigs-will-not-be-used-in-medical-schools-in-us-university-of-tennessee_n_577c05a7e4b09b4c43c15247